Gas-tight flask for the concurrent measurement of gas metabolism and growth in methane-oxidizing bacteria.
نویسندگان
چکیده
A flask is described for use in conjunction with a gas chromatograph and a spectrophotometer to follow methane and oxygen uptake, carbon dioxide production, and cell growth concurrently in methane-oxidizing cultures.
منابع مشابه
Hydrogen Oxidizing Bacteria as Poly(hydroxybutyrate) Producers
Batch culture of Ralstonia eutropha using the recycled gas closed circuit culture system was conducted in order to develop a practical fermentation system for industrial autotrophic culture for poly (hydroxybutrate) production. The gas phase of the culture system consisted of substrate gas so that gases in this culture could be recycled as long as the amount of the gas consumed would be repleni...
متن کاملExperimental Measurement of Methane and Ethane Mole Fractions during Gas Hydrate Formation
The super-saturation condition has to be provided to form gas hydrates. Consequently, the prediction of the guest molecule fraction in the aqueous phase is crucial in the study of the gas hydrate kinetics. In the present work, several experiments were carried out in a semi-batch reactor in order to determine the mole fraction of methane and ethane during gas hydrates formation (growth stage...
متن کاملAtmospheric Nitrogen Fixation by Methane-oxidizing Bacteria.
Davis, J. B. (Socony Mobil Oil Co., Inc., Dallas, Tex.), V. F. Coty, and J. P. Stanley. Atmospheric nitrogen fixation by methane-oxidizing bacteria. J. Bacteriol. 88:468-472. 1964.-Methane-oxidizing bacteria capable of fixing atmospheric nitrogen were isolated from garden soil, pond mud, oil field soil, and soil exposed to natural gas, indicating a rather wide prevalence in nature. This may exp...
متن کاملOn-Line Measurement of Dissolved Methane Concentration During Methane Fermentation in a Loop Bioreactor
A dissolved methane sensor based on silicone tube was designed, constructed and optimized.The silicone tube diameter, silicone tube length and helium flow rate (as the carrier gas(were considered as process parameters to be optimized.A continuous stream of helium (50 mL/min) was directed through the tubing, sweeping out the...
متن کاملEstimation of methane emission from the risers of urban gas network in the metropolis of Mashhad and evaluation of its economic and environmental effects
Energy Information Administration (EIA). 2022. Natural gas explained. https://www.eia.gov/energyexplained/natural-gas/use-of-natural-gas.php#:~:text=The%20United%20States%20used%20about,of%20U.S.%20total%20energy%20consumption Energy Information Administration (EIA). 2022. Natural Gas Consumption by End Use. https://www.eia.gov/dnav/ng/ng_cons_sum_dcu_nus_a.html IEA. 2020. Gas 2020. https:/...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied microbiology
دوره 28 3 شماره
صفحات -
تاریخ انتشار 1974